Now that you have everything you need to start growing, the cultivation journey of Psilocybe Cubensis mushrooms begins! We’ve split the process into 6 Units. You start by germinating your mushroom spores, and then transferring the germinated mycelium into liquid culture jars. Once enough liquid mycelium has grown, you then transfer some of this liquid mycelium into jars of sterile grain spawn. These jars colonize, and are then mixed with bulk substrate in a plastic tub, called a monotub. The bulk substrate takes about ten days to colonize and then starts fruiting. Finally, after the first flush is harvested, several more flushes may emerge.
In this module we walk through the steps required to make Agar mixed with nutrients such as honey or light malt extract powder. The nutrients provide food for the mycelium., sterelize it and then pour it into Dishes made out of plastic or glass which allow for sterilization and storage of nutrient agar. Mycelium is then grown in these dishes. in a clean environment, under the Still Air Box. The aim is to do this without contaminating the agar dishes in the process.
This module dives into the process of making and sterilizing liquid culture, using mason jars and lids which we modified especially for this purpose. Once sterilized, we will go into inoculating the An acronym for Liquid Culture – a liquid growth medium for mycelium, usually gown in a jar of sterile nutrient and water. with live mycelium so we can use it for further inoculation of substrate.
This module covers how to prepare, hydrate, sterilize and then inoculate popcorn grain spawn with live mycelium which was grown in the previous step. Again, our modified mason jar lids come to play in this step, with the custom injection ports we created.